Methodology for retroviral vector insertions in cancer research.

In an effort to help maximize the utility of the zebrafish as a model organism, Hopkins and colleagues set out to develop a large-scale insertional mutagenesis method. Although large-scale chemical mutagenesis screens were getting underway in several zebrafish labs at the time, there were concerns in the community at the time that identifying the molecular lesions using positional cloning methods would be inefficient. Hopkins believed that insertional mutagenesis, which had been very successfully employed in invertebrate model organisms would provide a valuable alternative or adjunct to the chemical screens.for zebrafish development . Also earlier research on gene expression in the bacterial virus lambda and on mouse RNA tumor viruses; cancer prevention & early detection.

Retroviral vectors & methods for production & use thereof; Method for retroviral vector insertions in fish

Category:
Biology- Molecular Biology

Completion Status:
yes

Patent Number:
Wo 00/56874; WO1996003034

Co-Inventor:
Wembiao Chen; Shawn Burgess; Adam Amsterdam; Nicholas Gaiano